Movie of single molecules of Zelda (green) tagged with mEos3.2 and histones (red) in a living drosophila embryo (nuclear cycles 13-14). The edge of the embryo is visible as the bright red line on the right side of the movie. Acquired at 100 ms exposure times.
Movie of nuclear divisions in the Drosophila embryo blastoderm stage visualized using halo-tagged Histone H2B labelled with JF549. Each frame/volume is 101 slices, spaced 200 nm apart for a total imaging volume of 51.2x51.2x20.2 (x,y,z) microns, there are 2.5 seconds between volumes with an exposure time of 10 ms at each slice. Imaged with a lattice light sheet microscope and rendered using volren MIP rendering in Amira.
Dynamics of Zelda spatial distribution in the context of an active transcription site imaged using the MS2 system (red) in a cycle 13 Drosophila embryo.
Video of my construction of the lattice light-sheet microscope in the Darzacq lab at UC Berkeley. Since this video we have made several modifications to the system including upgrading the cameras, adding a photoactivation laser independent of the imaging lasers, and some simplifications of the design to improve efficiency.